Accession Number:

ADA261021

Title:

Plasmid Stabilization to Insure Gene Expression

Descriptive Note:

Corporate Author:

MASSACHUSETTS INST OF TECH CAMBRIDGE DEPT OF BIOLOGY

Personal Author(s):

Report Date:

1992-10-10

Pagination or Media Count:

11.0

Abstract:

All unstable structurally plasmid in Bacillus subtilis was stabilized by modifying the nutritional environment and reducing the size of the plasmid. The stabilization was explained solely by the effect of the growth rate ratio between cells containing modified and parental plasmids. The recombinant protein produced by the B. subtilis system was Beta-galactosidase from Escherichia coli with the success achieved here, attention was shifted to cellulase production in Clostridium thermocellum including enzyme isolation and gene cloning.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE