DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
ADA259832
Title:
Post-Fusion Membrane Reorganization.
Descriptive Note:
Final rept. 28 Oct 91-31 Jan 93,
Corporate Author:
MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE
Report Date:
1993-01-27
Pagination or Media Count:
9.0
Abstract:
We have previously shown by thin section electron microscopy BJ 60 1037 in a contact zone induced between erythrocyte ghosts by dielectrophoresis, the use of an electrofusion protocol converts the contact zone into a fusion zone FZ containing from 1-225 fusion pores per micrometer squared. Also, after creation, both the FZ stability, and the time-dependent diameter expansion rates of the fusion zone are strongly dependent on an intact spectrin network. In the present project, and using computer-assisted analysis on video-recorded phase optics images of fusion zone diameters, we have found that the FZ diameter vs. time kinetics has two, or three, distinct phases I-III in erythrocyte ghosts with an intact or heat-disrupted 42 oC, 20 min, respectively, spectrin network. These phases, revealed by FZ diameter vs. time measurements under some combinations of various electric pulse parameters, temperatures 4-33 deg C during measurements, heat treatment temperatures 39-50 deg C, and dielectrophoretic force E sub ac 3.25, 4.25, and 5.25 Vmm, and under certain conditions, were remarkably independent from one another, yet the durations of Phase I 1.0-1.2 sec and Phase II 4.0 sec were remarkably invariant regardless of the variable studied. This suggested the existence of a complex but dissectable interplay of biomechanical factors....Membranes, Cells, Electrofusion, Electroporation, Red cells, Erythrocytes.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
