The Effect of Hyperbaric Oxygen and Pentoxifylline on the Rate of Neovascularization in Mice
Annual technical rept. Feb 1990-Feb 1992
ARMED FORCES INST OF PATHOLOGY WASHINGTON DC
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A polyvinyl alcohol sponge was implanted in mouse subcutaneous tissue to investigate two treatments INTERMITTENT HYPEROXIA 100 OXYGEN FOR 90 MINS TWICE A DAY AT 250 KPa and epidermal growth factor EGF which may modulate fibroblast infiltration. Two conditions were established for treatment exposure of animals to chronic hypoxia 12 oxygen for 23 hrday, simulating low oxygen tensions in problem wounds, and normoxia 21 oxygen. In experiments evaluating EGF, sponges were implanted whose core contained EGF covered with a slow release polymer, the other group with placebo. Sponges were harvested at 15, 25, and 32 days after implantation. The area of the disc infiltrated by fibroblasts was measured by planimetry. After 32 days exposure to hypoxic conditions 7 days before sponge implantation and 25 days after EGF slightly increased NS the area of fibroblast infiltration compared to placebo under both hypoxic and normoxic conditions. No significant differences were observed between the hypoxically conditioned groups and normoxic controls. Neither chronic hypoxia alone nor chronic hypoxia with intermittent hyperbaric oxygen administered 21-32 days after disc implantation affected the area of fibroblast infiltration. EGF significantly increased the area of the fibrous capsule around small PVA sponges after 15 days under normoxic conditions. Fibroblast-Hyperbaric Oxygen-Hypoxia- Polyvinyl Alcohol Sponge Wound.
- Medicine and Medical Research