Biophysical and Biochemical Mechanisms in Synaptic Transmitter Release.
Final rept. 1 Feb 1989-31 Jan 1992,
NEW YORK UNIV MEDICAL CENTER NY DEPT OF PHYSIOLOGY AND BIOPHYSICS
Pagination or Media Count:
The initial question addressed in 1989 was that of synaptic vesicle movement as determined by direct microscopic visualization. This research demonstrated that vesicles were actually mobilized from the point of injection in the axon to the active zones, i.e. the place where synaptic transmitter is released. It was also found that a change in either oxygenation or the surface properties of vesicles can lead to no movement or, to change in movement direction. The second aspect of synapse work performed that year was a demonstration of the category of calcium channel that is responsible for transmitter release. The work in 1990 demonstrated that miniature potentials could be modulated in the squid synapse by injection of Synapsin I and of protein kinase II. In the third year of the grant, 1991, the first demonstration of calcium microdomains in synaptic transmission was performed.