Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Plasmodium vivax-VK247 Sporozoites
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
Pagination or Media Count:
An enzymed-linked immunosorbent assay ELISA for the Plasmodium vivax-VK247 variant circumsporozoite CS protein was developed and evaluated using sporozoites produced by feeding mosquitoes on Thai patients with parasitologically confirmed P. vivax infections. The ELISA had a detection threshold of fewer than 50 sporozoites. Using this assay in conjunction with an ELISA for the VK210 polymorph, nearly 16 of the 235 P. vivax cases produced sporozoites positive only for the variant 69 produced sporozoites positive only in the VK210 assay and 15 were positive in both assays, indicating mixed infections. Twelve cases 5 produced sporozoites negative-in one assay and with unexpectedly low activity in the other ELISA, indicating the possibility of other CS protein polymorphs. Plasmodium vivax, circumsporozoites protein detection ELISA.