Use of Protein A as the Primary Layer in Fluorescent Microsphere Technology.
Final rept. Jun-Sep 1991,
CHEMICAL RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD
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Six unique monoclonal antibodies mAb to the galactose-binding adherence lectin of Entamoeba histolytica were assessed for their abilities to bind protein A, using BIAcore. Of the six antibodies tested, mAb H85, specific for pathogenic strains, showed the greatest degree of binding to Protein A. Therefore, protein A was used as a surface layer on microspheres to which mAbs H85 and 3F4 were attached. Conversion of this method to microsphere agglutination and enzyme linked immunabsorbent assay ELISA formats proved successful. Instrumented reads of agglutination reactions in the ELISA format achieved a lower limit of detection equivalent to 500 amoeba when performed on solubilized amebic antigen. Protein A, Fluorescence, Epitope, Adherence lectin, Microsphere, Monoclonal antibody.
- Anatomy and Physiology