Autoradiographic Distribution and Applied Pharmacological Characteristics of Dextromethorphan and Related Antitissue/Anticonvulsant Drugs and Novel Analogs.
Annual rept. 15 Jul 1991-14 Jul 1992,
LONDON UNIV (UNITED KINGDOM) DEPT OF PHARMACOLOGY
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Binding of dextromethorphan and its analogues to the dextromethorphan binding site and to the PCP and glycine binding sites associated with the N- methyl-D-aspartate receptor-channel complex was investigated using 3H-DM, H-TCP and 3H-glycine as radioligands. The experiments were carried out in 1m1 tubes miniblocks, in a final incubation volume of 0.5m1. All reagents were diluted and dispensed using a TECAN and filtering was done using a Brandel M-48R. Samples were counted in 5m1 of Esoscint A. after an extraction period of at least 6 hours, using a Beckman LS1701 liquid scintillation counter. All compounds were dissolved in distilled water except compound 3 which was dissolved in DMSOlactic acid and diluted with distilled water. Standards were obtained from commercial sources except dichlorokynurenic acid DCK which was a gift from Pfizer Central Research, Sandwich, Kent. Drugs, RAIII, Anticonvulsants, Antitissues, Dextromethorphan, Autoradiography, Pharmacokinetics.
- Anatomy and Physiology