Structure and Characterization of Nicotinic Acetylcholine Receptors from Peripheral and Central Nervous Systems.
Final rept. 1 Jul 88-30 Jun 90,
MINNESOTA UNIV MINNEAPOLIS DEPT OF CHEMISTRY
Pagination or Media Count:
In this section we describe studies of the long term stability of the receptor subunits in highly enriched membrane fractions purified by sucrose density gradient centrifugation followed by brief treatment at pH 11.0 to extract peripheral membrane proteins. This preparation represents the most highly purified membrane preparation available form any source. In addition we describe the purification of a proteolytic enzyme which is present in both unfractionated membranes in sucrose density gradient fractions enriched in the AcChR and to a lesser extent in th pH 11.0 treated membranes. The last part of this section deals with studies designed to determine whether long-term incubation with cholinergic agonists affects the functional states of the AcChR. For this purpose we describe experiments designed to determine whether the receptors ability to reversibly undergo changes between the resting state and the densensitized state is affected by incubation with agonist.