The Synthesis of Chemiluminescent Flavoabzymes and Their Use in Metal Ion Detection.
Final rept. 15 Mar 1990-15 Sep 1992,
CALIFORNIA UNIV SANTA BARBARA DEPT OF CHEMISTRY
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The ultimate goal of this research is to develop chemiluminescent CL abzymes and a technology for their use in metal ion detection. Four objectives must be reached in sequential order.1st, a molecule hapten which resembles in space requirements an intermediate in a CL reaction must be synthesized 2nd, a hapten protein conjugate antigen must be prepared and used to obtain monoclonal antibodies Mabs 3rd, one or more of the Mabs must catalyze the given CL reaction and .4th , the hapten must be-modifiable such that the CL Mabs become specific complexers of metal ions. Accomplishment of the 1st objective would provide the first Mab mimic of bioluminescence. Attempts to synthesize a hapten to generate Cl flavoabzymes will be discussed as will progress in the synthesis of a hapten to be used to prepare abzymes to catalyze the CL reaction of H202 with oxalate esters Monoclonal antibodies Mabs Abzymes hapten antigen chemiluminescence CL bioluminescence.
- Organic Chemistry
- Radiation and Nuclear Chemistry