Bentonite Clay Adsorption Procedure for Concentrating Enteroviruses from Water.
Technical rept. Jun 72-Jun 76,
ARMY BIOMEDICAL RESEARCH AND DEVELOPMENT LAB FORT DETRICK MD
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A method of adsorbing enteroviruses to bentonite clay was developed for use as a concentration technique designed to sample low levels of virus in environmental and tap waters. A divalentcation such as calcium was required to enhance the adsorption of large quantities of poliovirus onto bentonite within a 20 minute contact period. A minimum bentonite level of 50 mgL was necessary to adsorb the virus and to still allow efficient recovery and reasonable sample - size collection onto Miliporetm AP25 prefilters. Virus elution from the clay concentrated on the AP25 prefilters was most efficient with a solution mixture containing glycine 0.1 M, EDTA 0.01 M, and 1 bovine serum at pH 11, although tryptose phosphate broth at pH 7.2 was favored for field sampling with long field storage or shipping requirements. A water pH range of 4-10 did not significantly alter the efficiency of virus recovery. Although best recovery was obtained from highly polished water, good recoveries were also found for tap and wastewater with virus recoveries of 4585. The technique was found to be very versatile and could be easily scaled up for larger sample volumes without a loss in recovery efficiency. Enterovirus, Virus Concentration, Adsorption, Elution, Bentonite, Virus Sampling.
- Physical Chemistry