Construction and Evaluation of a Polyvalent Genetically Engineered Vaccine Candidate for VEE.
Annual rept. 3 Jun 91-29 Jun 92,
NORTH CAROLINA UNIV AT CHAPEL HILL DEPT OF MICROBIOLOGY AND IMMUNOLOGY
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We have shown previously that combining multiple independently attenuating mutations in a single Venezuelan equine encephalitis virus VEE strain using recombinant DNA techniques is a feasible strategy for producing a stable, immunogenic live virus vaccine. To improve the initial vaccine candidate, two major areas have been pursued. First, the number of attenuating loci from which the constituent mutations will be selected has -been expanded by further study of rapid penetration mutants, the investigational VEE vaccine strain, TC-83, and regions conserved among alphaviruses. Second, testing of alternate mutations at known attenuating loci has been initiated. Attenuating mutations have been compared in different strains of mice, and in a single strain infected by two different routes. These comparisons will aid in the selection of the two or three c t mutations of the improved vaccine candidate. A shuttle vector has been constructed to facilitate the combination of different glycoprotein mutations with mutations in other regions of the genome.
- Medicine and Medical Research