Evaluation of Hepatitis B Virus Photoinactivation in Serum and Cellular Blood Components by the Polymerase Chain Reaction.
AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH
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The purpose of this study was to investigate the usefulness of the polymerase chain reaction as a tool in the photoinactivation of transfusion transmitted viruses. There are currently two major methods of inactivating viruses in blood components. One is an oxygen dependent, membrane directed method and the other is a nucleic acid directed method. Current methods of evaluating photoinactivation involve either viral cultures or chimpanzee infectivity studies. These evaluation methods require from one week to one year to obtain results. The polymerase chain reaction amplifies a region of the viral deoxyribonucleic acid by repeated denaturing-annealing-extending of that region. If viral deoxynucleic acid is inactivated by crosslinking in the nucleic acid- directed inactivation procedure, the denaturation step can not proceed and previously positive results, using the polymerase chain reaction, will now be negative. This study used the polymerase chain reaction to evaluate the inactivation of hepatitis B virus with psoralen compounds in a nucleic acid- directed procedure.
- Medicine and Medical Research