Development of an Aquatic Bioassay Using the Medaka (Oryzias latipes) to Assess Human Health Risks: Tumor Immunodiagnosis.
Midterm rept. 28 Sep 90-27 Mar 92,
JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE
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In a continuation project for the development of aquatic bioassays as alternatives for carcinogenicity and toxicity testing to assess human health risk, immunohistochemical techniques IHC for the diagnosis of carcinogen induced neoplasms and other proliferative lesions in fish will be developed. Groups of medaka Oryzias latipes have been exposed to either N-methyl-n-nitro- n-nitroso-guanidine MNNG or methylazoxymethanol acetate MAM-Ac induce variable neoplasms and proliferative lesions to which IHC can be applied to identify differentiation antigens. Both peroxidase-antiperoxidase and avidin biotin complex technique are used to identify intermediate filament proteins keratin, desmin, vimentin, glial fibrillary acidic protein, and neurofilament protein. Other selected antigens may also be tested for based on histology. These findings will be correlated with histology, and ultrastructure. The results of this proposal will identify associations between cell types in neoplasms and in proliferative lesions of uncertain origin which cannot yet be typed by morphology alone in the fish, and thereby identify the possible progression of these lesions.
- Medicine and Medical Research