Molecular Recognition of Alpha-Neurotoxins
Final rept. 15 Mar 1989-15 Aug 1992
BAYLOR COLL OF MEDICINE HOUSTON TX DEPT OF BIOCHEMISTRY
Pagination or Media Count:
Peptides representing all the loops and surface regions of a- bungarotoxin were synthesized. Control peptide analogs were also prepared in which these sequences were randomized. The peptides were used to map the profiles of the antibodies and T cells obtained after BgTX immunization. Also, the abilities of anti-peptide antibodies and T cells to recognize the immunizing peptide and BgTX were determined. Immunization of mice with the individual peptides afforded significant protection against BgTX challenge. The most protective peptides, Ll, L2 and C-tail, allowed the mice to survive three times the LD50 of the controls i.e., Protective Index 3. Immunization with an equimolar mixture of the three peptides gave better protection P.I. 4.6, while an ovalbumin conjugate carrying these three peptides simultaneously afforded enormous protection P.I. 18.1 which was much higher than the protection by BgTX immunization P.I. 9.7. It is concluded that the multi- peptide conjugate should serve as an excellent vaccine against BgTX. The work is being prepared for a patent application. RA 1 Synthetic peptides Alpha- neurotoxins.