Biodegradation of Polychlorinated Methanes in Methanogenic Systems
Annual rept. 1 Dec 1988-1 Mar 1989,
ILLINOIS UNIV AT URBANA DEPT OF CIVIL ENGINEERING
Pagination or Media Count:
This research investigated biodegradation of several halogenated methanes under methanogenic conditions. Biodegradation of dichloromethane to C02 and acetic acid both environmentally acceptable products was demonstrated in a fixed-film reactor, operated at 20 deg C, a residence time as low as 0.25 day, and an influent concentration of 91 micro M. The biodegradability of chloroform was examined in a dichloromethane-degrading enrichment culture. Sustained consumption of chloroform approximately 8.5 micron M was achieved only when vitamin B12 was also added. Initially, equimolar amounts of chloroform and B12 were added when the amount of B12 added was gradually decreased to zero, chloroform degradation continued at the same rapid rate. Biodegradation of 14C chloroform yielded approximately 77 C02, 10 carbon monoxide, 7 soluble compounds about 15 of which consisted of acetate, and 2 nonsoluble compounds no 14CH4 was formed. Brominated methanes dibromomethane, bromochloromethane, and bromomethane degraded much more slowly if at all, than dichloromethane, they inhibited methanogenesis and dichloromethane degradation. Sustained degradation of chloromethane was demonstrated for an extended period without the need for an electron donor. Preliminary results suggest chloromethane serves as an electron donor under methanogenic conditions, just as dichloromethane does. Biotransformation of carbon tetrachloride was also demonstrated in the dichloromethane-degrading enrichment culture.
- Physical Chemistry