Mouse Model for Exoerythrocytic Stages of Plasmodium Falciparum Malaria Parasite,
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
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Research on the exoerythrocytic EE stages of human malaria parasites has been hindered because of the lack of an easily available suitable animal-mode. We report here an approach to produce mature EE-stage Plasmodium falciparum parasites by using severe combined immuno-deficient scid mice with transplanted human hepatocytes. Transplantation of human hepatocytes into scid mice scid hu-hep, their subsequent intravenous infection with P. falciparum sporozoites, and the development of mature liver-stage macrozoites was achieved. Immunofluorescent staining of scid hu-hep kidney tissue sections demonstrated the presence of circumsporozoite protein early during infection, merozoite surface antigen 1, and liver schizont antigen 1. The scid hu-hep model can serve as a source of human malaria liver-stage parasites, decreasing the need for nonhuman primates. Use of this model will facilitate characterization of EE- stage antigens and the assessment of stage-specific chemotherapeutic agents and candidate vaccines.
- Medicine and Medical Research