Carboxylesterases of the Testes: Role In Activation of Toxicants.
Final rept. 1 Dec 88-31 May 90,
AMERICAN COLL OF TOXICOLOGY BETHESDA MD
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Organ specific distribution of carboxylesterases Western blotting was determined to be liver lung testes fat pancreas kidney. Carboxylesterase distribution among cell types of the testes was examined by in situ hybridization techniques. Results were inconclusive, as both the probe and the control hybridized to tissues macromolecules. More refinement of this techniques should provide better results. Other accomplishments include examination of the down-regulation of carboxylesterase levels by glucocorticoids. Apparently esterase levels are most dramatically down-regulated approximately 6-fold by dexamethasone phosphate 60 mgkg x 5 days, i.p. in the testes compared to the other tissues containing this enzyme.