Hydrogen/Sulfur Autotrophy in the Hyperthermophilic Archaebacterium Pyrodictium brockii
Final rept. Jan 1989-Jan 1992
JOHNS HOPKINS UNIV BALTIMORE MD DEPT OF CHEMICAL ENGINEERING
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This work focused on the elucidation of physiological aspects of the hyperthermophilic archaea Pyrodictium brockii and Pyrococcus furiosus. Continuous cultivation methods were developed for both organisms to facilitate metabolic studies. For P. furiosus, we demonstrated the bioenergetic importance of a novel glycolytic pathway centered around a previously reported tungsten- requiring oxido-reductase. We also showed that sulfur reduction is implicated in P. furiosus bioenergetics and examined this issue in maltose-limited cultures. Patterns of regulation were followed through the induction of several enzyme activities involved in the organisms energetics. hyperthermophiles, microbial physiology.
- Physical Chemistry