Cellular and Molecular Level Responses After Radiofrequency Radiation Exposure, Alone or in Combination with X-Rays or Chemicals.
Annual technical rept. 1 Apr 1991-31 Mar 1992,
TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
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The focus of the first years activities, at a time when a major recruitment of personnel was underway, revolved around selection of the appropriate cell line for performing the mammalian cell mutagenicity studies preliminary studies with the Balbc 3T3 cell transformation assay mutation spectrum analysis of the spontaneous mutants arising in AS52 and BH4 chinese hamster ovary CHO cell lines liposome encapsulation studies of the polymer diazoluminomelanin DALM and redesign of the thermal control system originally constructed as a prototype for the Radiofrequency Radiation Division at USAF armstrong Laboratory. Most importantly, although the original proposal called for the study of the possible mutagenic interaction of microwaves and ionizing radiation using the AS52 line of CHO cells, the results of the mutation spectrum analysis study led to the decision to alter future protocols to perform these studies with the BH4 cells otherwise, the experiments would predominantly be measuring small deletion type mutations, and would not have enough sensitivity to pick up alterations of other types of mutation which might occur due to the microwave exposures. In addition, the liposome studies revealed the difficulty of encapsulating chemically synthesized DALM in this biological system these studies will need to be furthered using other resources. The redesign and construction of a modified thermal control system was begun, allowing for one control unit to perform the temperature measurement and temperature control functions in two separate incubator systems.
- Polymer Chemistry
- Nuclear Physics and Elementary Particle Physics