Immunologic Intervention in HIV Infection: Anti-Polymerase Responses and Hormonal Regulation.
Midterm rept. 17 Aug 90-16 Feb 92,
CORNELL UNIV MEDICAL COLL (WEILL) NEW YORK
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10-mers using a single amino acid overlap have been prepared from the three immunogenic domains of HIV-1 Pol which correlate best with reverse transcriptase activity amino acids 144-191, 214-335, 511-536. These 205 overlapping decapeptides were incorporated onto pins using technology developed by Cambridge Research Biochemicals, and utilized in ELISA assays with serum samples from HIV individuals at various clinical stages of HIV disease. Several discrete epitopes have been identified which correlate not only with capacity to block the catalytic activity of HIV RT, but also correspond to regions identified as inhibitory using murine anti-Pol monoclonal antibodies. RAI AIDS Rodents Immunotherapy Viral Activators Cellular Immunity Trans-Activation.
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