Carboxyalkylated Hemoglobin as a Potential Blood Substitute.
Final rept. 1 Sep 88-31 Oct 91, Annual rept. 1 Sep 90-31 Oct 91,
ROCKEFELLER INST NEW YORK
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The major focus has been on the preparation and full characterization of a new hemoglobin crosslinker - 2,5-diisothiocyanatobenzene sulfonic acid. Collaborative studies with investigators at the Letterman Army Institute of Research indicated that carboxymethylated hemoglobin was cleared from the circulation of rats with a half time of 42 minutes. The pseudo-first order plot indicates that a homogeneous population of molecules was present. In addition, there was no adverse pathological findings. The plasma retention time of the DIBS-crosslinked material that we prepared was also determined at Letterman to be 3-4 times longer than that of carboxymethylated hemoglobin. Cross-linking agents to produce derivatives of molecular weight 128,000 were also evaluated during this period. Other carboxyalkylating agents such as succinic semialdehyde in the presence of sodium cyanoborohydride have begun to be evaluated and compared with the carboxymethylated derivative. RA 2, Cross-Linking Agents, Carboxymethylation, Oxygen Affinity.
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- Medicine and Medical Research