Pathogenesis of DIC in Strain 13 Guinea Pigs Infected with Pichinde Virus.
Final rept. 1 Dec 88-30 Nov 91,
TENNESSEE UNIV MEDICAL CENTER AT KNOXVILLE DEPT OF MEDICAL BIOLOGY
Pagination or Media Count:
Pichinde virus infection of inbred guinea pigs is a model for arenaviral infections in humans. Infected animals experience reduced activity of multiple coagulation factors caused be either DIC or impaired factor synthesis due to liver injury. An RIA of guinea pig fibrinopeptide A gFPA has been developed to measure the degree of thrombin action in vivo. gFPA was synthesized via the solid-phase method and conjugated to BSA. High titer avid antisera were prepared in rabbit against gFPA-BSA conjugate. A double antibody RIA was establised employing goat anti-rabbit IgG to precipitate the primary complex composed of I-12-Tyr-gFPA and rabbit anti-gFPA-BSA. The working range for gFPA was between 1 ngm1 and 1 ugm1. The immunologically cross-reactive material, i. e., fibrinogen and FDP, was removed by mixing the plasma with ethanol ratio 13 and centrifuging the precipitate. Plasma gFPA immunoreactivities of outbred guinea pigs averaged 6.56 nqm1.
- Medicine and Medical Research