Development of an Antibody that Binds Sulfur Mustard
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
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An antibody that binds bis2-chloroethylsulfide sulfur mustard was developed. The immunizing antigen was prepared from the hapten 4-2-chloroethyl benzoic acid covalently bound to keyhole limpet hemocyanin KLH. The antibody was monitored by a solid phase enzyme-linked immunosorbent assay ELISA. The test antigen consisted of a second hapten, 8-chlorocaprylic acid, covalently bound to bovine serum albumin BSA. The test antigen was absorbed to the wells of 96-well plates. The immunizing and test antigens contain a common chloroethyl moiety. Thiodiglycol, the principal hydrolysis product of sulfur mustard, does not react with the antibody. This antibody, because of its specificity, has the potential to be a valuable tool for mustard research and forensic detection. Sulfur mustard, sulfur mustard antibody, antibody inhibition haptens.