Accession Number:

ADA250743

Title:

Development of an Antibody that Binds Sulfur Mustard

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Report Date:

1992-01-01

Pagination or Media Count:

7.0

Abstract:

An antibody that binds bis2-chloroethylsulfide sulfur mustard was developed. The immunizing antigen was prepared from the hapten 4-2-chloroethyl benzoic acid covalently bound to keyhole limpet hemocyanin KLH. The antibody was monitored by a solid phase enzyme-linked immunosorbent assay ELISA. The test antigen consisted of a second hapten, 8-chlorocaprylic acid, covalently bound to bovine serum albumin BSA. The test antigen was absorbed to the wells of 96-well plates. The immunizing and test antigens contain a common chloroethyl moiety. Thiodiglycol, the principal hydrolysis product of sulfur mustard, does not react with the antibody. This antibody, because of its specificity, has the potential to be a valuable tool for mustard research and forensic detection. Sulfur mustard, sulfur mustard antibody, antibody inhibition haptens.

Subject Categories:

  • Biochemistry
  • Toxicology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE