Accession Number:

ADA248892

Title:

Translational Regulation of Cloned Genes

Descriptive Note:

Final rept.

Corporate Author:

ARMY RESEARCH LAB RESEARCH TRIANGLE PARK NC ARMY RESEARCH OFFICE

Personal Author(s):

Report Date:

1992-02-04

Pagination or Media Count:

30.0

Abstract:

The actors controlling translational efficiency of mRNA in yeast were studied to aid in design of more efficient vectors for producing heterologous proteins in yeast. A plasmid was constructed with heterologous gene production driven by the yeast CUPI promoter and the killer virus M segment translation initiation signal. Although capped RNAs were more efficiently translated in vitro than uncapped RNAs of the same sequence, changes in the 5 untranslated leader rendered uncapped RNA as translatable as wild-type capped RNA. This result may make cytoplasmic RNA-based cloning vectors feasible in yeast. Yeast, Recombinant DNA gene expression, Translation, Transcription, Polymerase chain reaction, mRNA Cap.

Subject Categories:

  • Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE