Accession Number:

ADA248508

Title:

Scientific Expert in Metagenesis of Francisella Tularensis (Vaccine Strain) to Produce Marked Strains

Descriptive Note:

Final rept. 15 May-15 Dec 1991

Corporate Author:

KENTUCKY UNIV LEXINGTON

Personal Author(s):

Report Date:

1991-12-13

Pagination or Media Count:

12.0

Abstract:

Final Report on Specific Tasks No. 1-5 of Contract Independently plan and perform procedures to select for spontaneous mutations in the 10-6 to 10-10 frequency range, that render F. tularensis live vaccine strain resistant to one or more antibiotics or metabolic poisons e.g., nalidixic acid, rifamycin, streptolydigin, azide that can easily be incorporated into bacteriological media. Independently plan and perform procedures to purify and store the mutants for further characterization. Independently plan and perform procedures to characterize the growth rates of the mutants both in the presence and absence of the antibiotic to which resistance has been obtained.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE