Molecular Ecology of Bacterial Populations in Environmental Hazardous Chemical Control
Final rept. 1 Dec 1988-30 Nov 1991,
TENNESSEE UNIV KNOXVILLE
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Basic research was conducted to develop and explore the use of modern molecular biology techniques in understanding the dynamics of microbial populations engaged in biodegradation of environmental pollutants. The research focused on 1 the use of environmental DNA extraction and gene probing techniques to quantify the presence and distribution of degradative genes in the environment, 2 characterizing new-degradative organisms and plasmids for eventual development of new catabolic gene probes for environmental use, and 3 construction of novel bioluminescent reporter bacteria to act as biosensors of catabolic activity in the environment.