Accession Number:

ADA247626

Title:

Recognition of DNA by EcoRI Restriction Endonuclease and Methylase

Descriptive Note:

Rept. for 1 Feb 1988-1 Feb 1991,

Corporate Author:

CALIFORNIA UNIV LOS ANGELES

Personal Author(s):

• Feigon, Juli

Report Date:

1992-01-07

Pagination or Media Count:

3.0

Abstract:

NMR Studies of Echinomycin-DNA Complexes include the interaction of echinomycin, a cyclic octadepsipeptide antibiotic, with DNA oligonucleotides. These complexes were studied by two-dimensional proton NMR techniques. Echinomycin binds in a sequence specific manner to DNA by bis-intercalation, with a preferred 4 base binding site centered on CpG. Crystal structures of echinomycin and the related Triostin A complexed to DNA hexamers and octamers solved in the Rich laboratory showed that base pairs adjacent to the echinomycin binding site were Hoogsteen rather than Watson-Crick base paired. However, the evidence for Hoogsteen base pairing in complexes of echinomycin with DNA restriction fragments has been equivocal Waring and Dervan laboratories. We have been studying the solution structures of echinomycin-DNA complexes in order to determine 1 if Hoogsteen base pairs are forming adjacent to echinomycin binding sites in solution and 2 the sequence specificity of echinomycin binding in terms of the structures formed.

Descriptors:

• *CRYSTAL STRUCTURE
• SITES
• STRUCTURES
• FRAGMENTS
• DEOXYRIBONUCLEIC ACIDS
• SOLUTIONS(GENERAL)
• RECOGNITION
• BINDERS
• TWO DIMENSIONAL
• PROTONS

Subject Categories:

• Biochemistry
• Pharmacology
• Organic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE