Accession Number:

ADA247289

Title:

Hepatic Metabolism of Perfluorinated Carboxylic Acids and Polychlorotrifluoroethylene: A Nuclear Magnetic Resonance Investigation in Vivo

Descriptive Note:

Annual technical rept. 15 Feb-14 Dec 1991

Corporate Author:

WRIGHT STATE UNIV DAYTON OH MAGNETIC RESONANCE LAB

Personal Author(s):

• Reo, Nicholas V.

Report Date:

1992-01-24

Pagination or Media Count:

11.0

Abstract:

This report describes recent results of studies designed to investigate the metabolic effects of perfluoro-carboxylic acids on liver carbohydrate and high-energy phosphorous metabolism. Carbon-13 nuclear magnetic resonance NMR spectroscopy was used in conjunction with 13 C isotope labeling to monitor the dynamic conversion of glucose to glycogen in rat liver in vivo. The results show that perfluoro-decanoic acid PFDA causes a marked inhibition in hepatic glycogen synthesis in rats at 3 days post treatment n5 and complete inhibition at 5 days post dose n5. Hepatic glucose and blood glucose levels are also slightly elevated within the first 15 min. following a glucose load in PFDA rats versus controls p0.05. Preliminary data reveal that glycogen synthesis from alanine via gluconeogenesis is functional in PFDA- treated rats. This suggests that the inhibition in glycogenesis from glucose may involve the transport of glucose into hepatocytes andor its phosphorylation by glucokinase. Further studies are in progress which investigate this hypothesis. In studies of the high-energy phosphorous metabolism in PFDA-treated rats. P31 NMR reveals normal ATP levels and an anomalous signal in the phosphomonoester region of the liver spectrum. The source of this phosphorous metabolite has not yet been identified and is the focus of ongoing research efforts. These studies are providing new information about the metabolic effects of perfluorocarboxylic acids and advancing the development of NMR techniques in the field of toxicology.

Descriptors:

• *METABOLISM
• *LIVER
• CONVERSION
• ANOMALIES
• RATS
• HIGH ENERGY
• SIGNALS
• DOSAGE
• IN VIVO ANALYSIS
• TOXICOLOGY
• CARBOXYLIC ACIDS
• FLUORINATION
• GLUCOSE
• METABOLITES
• BIOSYNTHESIS
• CARBOHYDRATES
• PHOSPHORUS
• CELLS(BIOLOGY)
• BLOOD CHEMISTRY
• GLYCOGEN
• REGENERATION(PHYSIOLOGY)
• ALANINES
• PHOSPHORYLATION
• BLOOD VOLUME
• ACIDS
• INHIBITION
• HYPOTHESES
• TRANSPORT
• SPECTRA
• SPECTROSCOPY
• SYNTHESIS
• DYNAMICS

Subject Categories:

• Biochemistry
• Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE