Accession Number:

ADA247285

Title:

Stain Test Modules for Periodontal Diagnosis

Descriptive Note:

Status rept. 1 Apr-30 Jun 1989,

Corporate Author:

NORTH CAROLINA UNIV AT CHAPEL HILL DENTAL RESEARCH CENTER

Personal Author(s):

Report Date:

1989-07-18

Pagination or Media Count:

42.0

Abstract:

Studies in our laboratories and clinics since the beginning of this contract with direct cytochemical methods for the light and electron microscopic demonstration and discrimination of neutrophils , B-lymphocytes and plasma cells and T-helper and suppressor cells have shown the utility of these methods in demonstrating active periodontal disease. A variation of the periodic acid- SchiffPAS method known as the PATS reaction has proven very important in positively staining the types of Gram negative bacteria present in subgingival plaques. At the end of last year it was noted that activated macrophages could be demonstrated in smears of subgingival plaque or crevicular fluid from periodontal disease sites by out PATS reaction as well as in gingival biopsy specimens as had previously been reported by others. The fact that these cells were macrophages, not neutrophils, was supported by their greater acid phosphatase, nonspecific esterase and proteolytic enzyme activities as well as their size, morphology and increased numbers at diseased sites shown by cytochemical methods for light and electron microscopy.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE