Analysis of Saxitoxin from Urine Using Dynamic FAB/MS
Final rept. 15 Jul 1989-14 Aug 1991,
MINNESOTA UNIV ST PAUL DEPT OF PLANT PATHOLOGY
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An improved method of saxitoxin analysis in urine using continuous Flow Fast Atom Bombardment Mass Spectrometry CFFABMS was developed. Parameters studied were matrix composition, matrix flow rate, temperature of probe tip, probe tip design and sample extraction. Optimal detection was obtained using the following matrix composition 5 glycerol, 0.5 acetic acid, 0.025 sodium dodecylsulfate, 0.1 polyethylene glycol PEG 400 and 0.5 PEG 300 probe tip temperature -55 C flow rate 5 or 8 microliter per min probe tip Olson-Hogge design. The STX standard was detected at 200 picograms with signal-to-noise ratio of 11. The percent recovery of saxitoxin from human urine after cleanup on a weak cation exchange CBA Bond-Elut Analytichem Inter. Varian, Anaheim, CA column was 75.