Glutamate/NMDA Receptor Ion-Channel Purification, Molecular Studies and Reconstitution into Stable Matrices
Final rept. 15 Mar 1988-1 Jul 1991
KANSAS UNIV LAWRENCE CENTER FOR BIOMEDICAL RESEARCH
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This is the final report of a project that had as its primary focus the isolation of the NMDA receptor protein complex and the reconstitution of these proteins for the development of biosensors. The progress made in all phases of this work has met the goals selected for the research. 1 Two proteins of the receptor were fully purified and characterized. 2 The intact complex was purified and biochemically characterized. 3 One of the protein CDNAs was cloned and sequenced. 4 The complex was reconstituted into liposomes and planar lipid bilayers. 5 A prototype sensor based on this receptor complex was developed. 6 Cell model systems for the study of the receptor were developed.
- Medicine and Medical Research