Neuropathological Consequences of Exposure to Sublethal Doses of Cyanide
Midterm rept. 29 Jan 1990-28 Jan 1991
KANSAS UNIV MEDICAL CENTER KANSAS CITY DEPT OF MEDICINE
Pagination or Media Count:
Experiments focused on the effects of cyanide on energy metabolism, brain edema and histology. Wistar rats were infused iv with NaCN infusion was started again. The local cerebral glucose use LCGU was measured by the deoxyglucose 2-DG procedure. A shift from aerobic to anaerobic metabolism and the oxidation-reduction state of the brain extracellular fluid ECF were determined by intracerebral microdialysis. Immediately after iv infusion of NaCN, there was a robust increase in glucose use. Also, there was a dramatic increase, up to 5-fold, in lactate in the ECF indicating an increase in anaerobic metabolism. Sixty min after NaCN, LCGU was markedly reduced in all 46 brain regions measured, except for choroid plexus. Thus, the expected increase in glucose use as a result of anaerobic metabolism appeared to be brief. The time course profile of the 2-DG in the ECF shows that glucose transport across the blood brain barrier was not inhibited by NaCN. In fact, there was a higher concentration of the 2-DG in the ECF at a time when the LCGU was extremely low.
- Medicine and Medical Research