Evaluation of the Initiation/Promotion Potential of CTFE Trimer Acid
Final rept. Mar 1989-Jul 1990
NSI TECHNOLOGY SERVICES CORP DAYTON OH
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CTFE trimer acid is a metabolite of the 6-carbon oligomer of Halocarbon 3.1 oil, a nonflammable hydraulic fluid composed of perhalogenated oligomers of varying chain length. Administration of CTFE trimer acid by oral gavage for 3 months resulted in a slight increase in the rate of peroxisomal beta-oxidation, but not relative liver weight. An increase in peroxisomal beta- oxidation has been correlated with the formation of hepatic tumors. Therefore, the present study was designed to evaluate CTFE trimer acid for both tumor initiation and promoting ability. Male Sprague-Dawley rats 4 weeks old were partially hepatectomized. Groups of animals received a single dose of CTFE trimer acid as the initiator, followed by chronic phenobarbital administration, a known tumor promotor, for either 3 or 9 months. Diethylnitrosamine, a known tumor initiator, was administered as a single dose to separate groups of animals that were administered different doses of CTFE trimer acid as a promotor for either 3 or 9 months. Quantitative sterological analysis was performed on foci from liver sections stained for a variety of histological and histochemical markers. CTFE trimer acid does not possess tumor initiation ability. However, a significant increase in focicm2 and focicm3 in livers of treated animals over those of control after 9 months of treatment clearly indicates that CTFE trimer acid has promoting activity. Interestingly, no increase in either beta-oxidation or relative liver weight was noted in animals that received CTFE trimer acid as a tumor promotor.
- Inorganic Chemistry