Accession Number:

ADA243424

Title:

Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes

Descriptive Note:

Annual rept. Oct 90-Oct 91,

Corporate Author:

PROGRAM RESOURCES INC FREDERICK MD

Personal Author(s):

Report Date:

1991-01-01

Pagination or Media Count:

67.0

Abstract:

There are three main objectives of the research. First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig- genes into human hematopoietic progenitors and then grow and activate those cells in an animal model.

Subject Categories:

  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE