Accession Number:
ADA243424
Title:
Transfection of Murine and Human Hematopoietic Progenitors with Rearranged Immunoglobulin Genes
Descriptive Note:
Annual rept. Oct 90-Oct 91,
Corporate Author:
PROGRAM RESOURCES INC FREDERICK MD
Personal Author(s):
Report Date:
1991-01-01
Pagination or Media Count:
67.0
Abstract:
There are three main objectives of the research. First to isolate hematopoietic stem cells in numbers adequate for transfection with rearranged immunoglobulin genes. Second, to develop techniques which will allow B cells expressing the transfected Ig-gene product to activated by anti-idiotypic antibodies so that high levels of serum antibody are produced. Finally, to develop a human-mouse chimera that will allow us to transfect rearranged Ig- genes into human hematopoietic progenitors and then grow and activate those cells in an animal model.
Descriptors:
Subject Categories:
- Microbiology