The Priming Effects of Tumor Necrosis Factor and Interleukin-1 on Canine Neutrophils Stimulated with Interleukin-8
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Interleukin 8 IL-8 is a novel neutrophil activating peptide that was recently identified in secretions from mononuclear phagocytes and endothelial cells. The purpose of this study was to determine the interplay between two well documented inflammatory mediators, Tumor Necrosis Factor TNF and Interleukin-1 IL-1, and IL-8 in the priming of neutrophils for increased functional activity. Neutrophil functional activity was measured using three laboratory assays. A vitamin B12 binding protein assay was used to detect content andor degranulation of secondary granules, a chemotaxis assay was used for migration studies, and a dichlorofluorescein DCF hydrogen peroxide production assay was used to assess the respiratory burst function of the cell.
- Medicine and Medical Research