Accession Number:

ADA243368

Title:

The Priming Effects of Tumor Necrosis Factor and Interleukin-1 on Canine Neutrophils Stimulated with Interleukin-8

Descriptive Note:

Master's thesis,

Corporate Author:

AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH

Personal Author(s):

Report Date:

1991-08-01

Pagination or Media Count:

87.0

Abstract:

Interleukin 8 IL-8 is a novel neutrophil activating peptide that was recently identified in secretions from mononuclear phagocytes and endothelial cells. The purpose of this study was to determine the interplay between two well documented inflammatory mediators, Tumor Necrosis Factor TNF and Interleukin-1 IL-1, and IL-8 in the priming of neutrophils for increased functional activity. Neutrophil functional activity was measured using three laboratory assays. A vitamin B12 binding protein assay was used to detect content andor degranulation of secondary granules, a chemotaxis assay was used for migration studies, and a dichlorofluorescein DCF hydrogen peroxide production assay was used to assess the respiratory burst function of the cell.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE