Accession Number:

ADA243279

Title:

Pathobiology of HIV in the Human Monocyte-Macrophage

Descriptive Note:

Annual rept. 28 Sep 1990-27 Sep 1991,

Corporate Author:

NEW ENGLAND DEACONESS HOSPITAL BOSTON MA

Personal Author(s):

Report Date:

1991-10-24

Pagination or Media Count:

22.0

Abstract:

In the past year, we have made considerable progress in addressing specific aims of our proposal. We have studied the interaction of HIV with monocyte-macrophages with particular emphasis on comparing these interactions with those that occur in human T lymphocytes. We have found that there are cellular transcription factors which interact with the HIV LTR independent of NF-kappa B which could be important in virus expression in different cells. The role of cytokines including the activity of such transcription factors is under investigation. We have also found that a newly identified cytokine, the kit ligand KLstem cell factor SCF, protects myeloid and erythroid progenitor cells from the suppressive effects of cytokines such as tumor necrosis factor. While KLSCF can amplify the proliferative response of hematopoietic progenitors, it does not increase HIV replication in target cells. KLSCF is an attractive candidate cytokine to be used therapeutically in patients with AIDS. During the first year project, we have also identified sequences within the negative regulatory element NRE of the HIV LTR which could interact with the family of transcription factors termed ARP. We are characterizing the expression of the ARP genes in different cells to determine whether they may influence viral tropism.

Subject Categories:

  • Biology
  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE