Development and Validation of Rapid In Situ Assays of Environmental Mutagenesis
Annual technical rept. 1 Jul 1989-30 Sep 1990,
OKLAHOMA STATE UNIV STILLWATER DEPT OF ZOOLOGY
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All field work at a site contaminated with polychlorinated biphenyls PCBs in Pryor, Oklahoma has been completed. Standard chromosome aberration assays on Peromyscus leucopus white-footed mouse, Sigmodon hispidus cotton rat, and Reithroduontomys fulvescens fulvous harvest mouse from the Pryor site and from three matched reference sites has been completed. All samples of spleen tissues for flow cytometric analyses FCM have been prepared. Trial runs for FCM analysis have been carried out and all FCM analyses should be completed by the end of the year. PCB tissue content analysis has been completed on seven animals randomly chosen from the Pryor sites and shows significant increases over background levels. Field analyses at a second site in Payne Country, Oklahoma contaminated with a mixture of radioactive and chemical wastes has been underway for one year. Data collection has involved monthly sampling for several demographic variables and tri-monthly sampling for cytogenetic and tissue residue variables. Field work for this year has been completed and slides are currently being analyzed. Laboratory validation experiments involving exposure to known levels of known clastogens have been initiated.
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