Acute Exposure of Medaka to Carcinogens: An Ultrastructural, Cytochemical and Morphometric Analysis of Liver and Kidney
Annual rept. 1 Feb 90-31 Jan 91,
SOUTHEASTERN LOUISIANA UNIV HAMMOND
Pagination or Media Count:
The primary objective of this project is to determine the ultrastructural and selected cytochemical effects of diethylnitrosamine DENA and trichloroethylene TCE on hepatic and renal tissues of the Japanese medaka, Oryzias latipes. Another critical phase of the investigation is to determine whether DENA can function as an inducer of cellular transformation for TCE. One group of 60 medaka was exposed to DENA 10mgL for 48hrs, rinsed and maintained in a tank of fresh water. Another group of 60 fish has been continuously exposed to TCE 10mgL. A third group of medaka was exposed to DENA 10mgL for 48hrs, rinsed in fresh water and subsequently has been continuously exposed to TCE 10mgL. A final group was placed in fresh water and served as a control. The sacrifices have been scheduled at 2,4,6 and 12 months subsequent to the initial exposure to DENA. At this point in the study, the first two sacrifices have been conducted and the tissues appropriately processed for electron microscopy. The specific enzymes selected for analysis by means of electron-dense markers are acid phosphatase, peroxidase and ATPase. Samples collected at the first two sacrifices have been processed.
- Medicine and Medical Research