Accession Number:

ADA242794

Title:

Location of Receptor-Binding Region of Protective Antigen from Bacillus anthracis

Descriptive Note:

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s):

Report Date:

1991-08-07

Pagination or Media Count:

15.0

Abstract:

The pag gene, which codes for protective antigen PA, a common component of the lethal and edema toxins of Bacillus anthracis, was cloned and express in Escherichia coli. Nested deletions of pag were generated into the C- terminus coding region. REcombinant proteins were analyzed by Western blot with either an anti PA polyclonal antisera or two monoclonal antibodies that neutralized lethal toxin and edema toxin activities by inhibiting the binding of PA to cell receptors. Localization of the receptor binding domain within the C- terminal region of PA was suggested by the inability of the monoclonal antibodies 3B6 and 14B7 to recognize the recombinant proteins expressed by C- terminal deletions of the pag gene.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE