Accession Number:

ADA242475

Title:

Development of Hematopoietic Growth Factors for Use in Military Personnel

Descriptive Note:

Status rept. 1 Mar-31 Jul 91,

Corporate Author:

NEW ENGLAND DEACONESS HOSPITAL BOSTON MA

Personal Author(s):

Report Date:

1991-07-12

Pagination or Media Count:

3.0

Abstract:

We have continued our work on the new series of erythropoietin mutants. Interestingly, in sequencing the first five mutants that we derived, we discovered that another mutation had occurred spontaneously. In the short term, this has resulted in some delay in our studies, since we now feel it necessary to sequence not only the mutated domain of each cDNA, but rather the entire cDNA to verify the absence of other spontaneous mutations. However, in the long term, it is to our benefit to realize that the state-of-the-art methodology that we have employed in preparing these mutants is subject to some technical artifacts. Discovering this at this early stage of our studies will save us many problems in the long run. We expect that within the next three to six months, we will have finished all of our sequencing and will begin to express some of these mutants and initiate their biological characterization.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE