Core Scientific Effort for Biosurfaces Studies. Task 1
Rept. for 1 Jun 1989-30 Aug 1991
STATE UNIV OF NEW YORK AT BUFFALO AMHERST
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The objectives for the 1991-92 year are, in concert with the IUCB 5- year plan, to expand the variables of the assays to include more biologically specific probes of the expression of particular conditioning film epitopes. The experimental plan anticipates the use of anti-albumin, anti globulin, anti fibrinogen and anti betalactoglobulin against spontaneously adsorbed thin films of the primary protein antigens on well characterized reference substrata. There also will be the production of specific, tailored substrata ocatadecyl amine, alcohol, and acid monolayers from both Langmuir-Blodgett transfer and the retraction process for examination of the competing hypotheses of dipole or charge based action at a distance hydrophobic hydrophilic ratio, and surface free energy as the major influences on specific bioadhesion. Particular emphasis will be given to the possible expression of cryptitopes previously hidden functional sites as a consequence of substratum-induced protein conformational changes. Polarized, internal reflection infrared analyses will be attempted to obtain these data directly, and collaboration will be sought with IUCB core program investigators using both specific staining and high vacuum analytical instrumental approaches for functional group identification.