Histology of Infection of Hydrilla verticillata by Macrophomina phaseolina
ARMY ENGINEER WATERWAYS EXPERIMENT STATION VICKSBURG MS ENVIRONMENTAL LAB
Pagination or Media Count:
An isolate of Macrophomina phaseolina Tassi Goid. has demonstrated potential as a boicontrol agent for the noxious submersed aquatic macrophyte Hydrilla verticillata L.F. Royle in recent studies. The purpose of this study was to follow the histology of infection of H. verticillata by M. phaseolina with particular attention to the chronology of attachment, penetration, and colonization during the early stages of pathogenesis. Hydrilla sprigs 10 cm were taken from outdoor ponds at the US Army Engineers Lewisville Aquatic Ecosystem Research Facility in Lewisville, TX. These were washed in distilled water and placed in 1.5- by 10-cm plastic petri dishes filled with 20 ml of sterile distilled . Cultures of M. phaseolina isolate FHy18 were grown for 7 days in 250-ml Erlenmeyer flasks with 100 ml of Richards V8 broth on a shaker at 150 rpm at 25 deg C. The Fungal cultures were blended in a Waring blender for 15 sec to produce a hyphal suspension for use as inoculum.