Anti-Idiotype Probes for Toxin Detection
Midterm rept. 15 Apr 1989-14 Apr 1991
KANSAS STATE UNIV MANHATTAN DIV OF BIOLOGY
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This report covers the progress for the first two years of the active grant. During this time we have developed methods to purify the exfoliative toxins A and B from culture supernatants. These toxins have been used for polyclonal and monoclonal antibody production. We have identified proteins on the cell surface of thymocytes that bind to exfoliative Toxin A. This protein is similar to the protein that is precipitated with antibody preparation to class Ii molecules. We have shown that several exotoxin of S. aureus stimulate tumoricidal activity in peritoneal macrophages. Tumor necrosi factor a was also produced in response to the toxins as was interleukin-6. The data suggest each toxin activates macrophages differently.