Accession Number:

ADA242097

Title:

Comparative Infectivity Determination of Dengue Virus Vaccine Candidates in Rhesus Monkeys, Mosquitoes, and Cell Culture

Descriptive Note:

Annual rept. 1 Sep 89-31 Aug 91,

Corporate Author:

PURDUE UNIV LAFAYETTE IN

Personal Author(s):

Report Date:

1991-08-28

Pagination or Media Count:

10.0

Abstract:

On February 12, 1991, sixteen rhesus monkeys were sent from the Caribbean Primate Center Sabana Seca to the Animal Resources Facilities at the Medical Sciences Campus. The monkeys were bled on February 12, and on February 26, and the sera were stored at -20 C. The Plaque Reduction Neutralization test PRNT and the hemagglutination inhibition HI test were performed using these serum samples Pre vaccination bleeds. The PRNT was performed using LLC-MK2 cells sent by Dr. Goro Kuno CDC, San Juan Laboratories grown in multiwell plates as described before. Vaccines and the other materials were received on 4 491. On April 22, 1991 groups of four monkeys were innoculated s.c. with 0.5 ml of each candidate vaccine. The titration of the four vaccine candidates were made on the same day of inoculation using LLC-MK2 cells.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE