Genotoxicity Assessment of Chlorotrifluoroethylene Tetramer Acid using a Battery of In Vitro and In Vivo/In Vitro Assays
Final rept. Oct 1989-Nov 1990
NSI TECHNOLOGY SERVICES CORP DAYTON OH
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Chlorotrifluoroethylene CTFE tetramer acid, the perhalogenated fatty acid metabolite of the eight-carbon oligomer of polychlorotrifluoroethylene pCTFE, was evaluated in in vitro bioassays to assess its potential genotoxic activity. The assays conducted were the Ames Salmonellamicrosomal mutagenicity assay, the Chinese hamster ovary CHO gene mutation assay, the CHOsister chromatid exchange SCE and chromosome aberrations assays, the BALBc-3T3 cell transformation assay and in vivoin vitro unscheduled DNA UDS and S-phase synthesis assays. CTFE tetramer acid did not demonstrate genotoxic potential in any of the in vitro assays and was also negative in the UDS assay. However, a small, but significant, dose-related increase in S-phase synthesis occurred 48 hours following oral administration of doses of CTFE tetramer acid ranging from 11 to 44 mgkg. This increase in S- phase synthesis indicated a rapid response to hepatotoxicity caused by CTFE tetramer acid but the results of the test battery would predict no genetic risk from exposure.
- Medicine and Medical Research