Salivary Platelet Activating Factor Levels in Periodontal Disease
AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH
Pagination or Media Count:
Platelet activating factor PAF, a potent phospholipid inflammatory mediator is found in normal human saliva. This study sought to evaluate possible relationships between salivary PAF levels and periodontal disease. One ml of mixed saliva was collected from 69 untreated subjects presenting for evaluation at the UTHSC dental hygiene or periodontal clinic. After phospholipid extraction and fractionation by thin layer chromatography, salivary PAF activity was determined by platelet bioassay. PAF activity, estimated relative to that of authentic PAF 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine C16O-AGEPC, was expressed in C16O-AGEPC fmole equivalentsml saliva tracer amounts of H3- AGEPC included in all samples prior to initial extraction were used to calculate PAF recovery. Subjects were subdivided into 6 similarly sized groups according to disease severity based on probing depths. The healthiest group, Gp 1, had or 4 mm probing depths throughout, while the most severely affected group, Gp 6, averaged 4 mm probing depths in 50 of the sites. Correlation was found between the number of bleeding sites and the 6 groups. PAF levels generally increased from Gp 1 to Gp 6, with 1 levels 2,365 or 900 C16O-AGEPC fmole equivalentsml being significantly lower than Gps 5 and 6 10,489 or 1,775 and 10,251 or 3,075, respectively. The findings indicate that salivary PAF levels correlate with periodontal status and suggest that this phospholipid inflammatory mediator may play a role in the pathogenesis of periodontal disease.
- Medicine and Medical Research