Production of Reactive Oxygen Species by Polyhalogenated Cyclic Hydrocarbons (PCH)
Annual rept. 15 Jun 90-14 Jul 91,
CREIGHTON UNIV HEALTH SCIENCES CENTER OMAHA NE
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We have developed a HPLC method for the simultaneous determination of four lipid peroxidation products, namely, formaldehyde, malondialdehyde, acetaldehyde and acetone. This procedure has wide-spread applicability to exposure to environmental pollutants as well as the study of various disease states. Initial studies have clearly demonstrated that the in vitro exposure of peritoneal macrophages, mitochondria and microsomes to selected PCH results in the production of reactive oxygen species as well as a decrease in membrane fluidity. Furthermore, the administration of a variety of polyhalogented cyclic hydrocarbons to rats results in an induction of DNA damage as assessed by the formation of DNA single strand breaks. The results support our basic hypothesis concerning the ability of polyhalogenated cyclic hydrocarbons to induce production of reactive oxygen species which may lead to membrane damage and the subsequent manifestation of toxic symptoms following exposures.
- DEOXYRIBONUCLEIC ACIDS
- IN VITRO ANALYSIS
- INDUCTION SYSTEMS