Accession Number:

ADA239093

Title:

DNA-Mediated Electron Transfer and Application to 'Biochip' Development.

Descriptive Note:

Final rept. Jun 88-May 91,

Corporate Author:

OREGON STATE UNIV CORVALLIS DEPT OF BIOCHEMISTRY AND BIOPHYSICS

Personal Author(s):

Report Date:

1991-07-25

Pagination or Media Count:

5.0

Abstract:

To study the electronic properties of double-stranded DNA as to determining whether this macromolecule can support electron transport processes. This pertains to possibly utilizing the base sequence and secondary structure of DNA as a matrix for developing molecular level electronic components. Toward these goals, we have studied the anisotropic electronic properties of DNA single crystals using reflectance spectroscopy and studied the interactions of transitions metals with double-stranded DNA by X-ray diffraction. We have also synthesized a number of porphyrin and acridine modified DNA molecules, and assembled a photoflash photolysis apparatus for direct study of electron transfer through DNA. In related work, we have shown that the propensity for DNA to adopt specific double helical conformations can be predicted from calculations of solvent accessible surfaces. From this, we were able to obtain diffraction quality single crystals of DNA oligomers in a predictive manner.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE