Trimester Report on Contract N00014-89-J-3073 (Massachusetts General Hospital)
Rept. for 15 Mar-12 Jul 91,
MASSACHUSETTS GENERAL HOSPITAL BOSTON
Pagination or Media Count:
Over the last several months we have especially focused on two projects in the laboratory related to discoveries we made early in the spring relating to 1 the interactions that LPS undergoes before binding to anti-LPS immunoglobulins in hyperimmune antisera to rough mutant E. coli J5, and 2 IgG titers to the O-polysaccharide of LPS in the screened plasma of normal blood donors. Both of these projects represent offshoots from our overall aim of purifying the substances in inflammatory serum which bind LPS. We completed the laborious task of radio labeling with tritium a panel of gram negative bacteria consisting of the 10 different strains of E. coli which most commonly cause infections. Each strain was grown in 100 mls broth containing 10-25 millicuries of 3H-acetate to radio label the bacteria.