Characterization by NMR and Fluorescence Spectroscopy of Differences in the Conformation of Non-Aged and Aged Organophosphoryl Conjugates of AChE
Final rept. 1 Sep 1987-30 Nov 1989
ISRAEL INST FOR BIOLOGICAL RESEARCH NESS ZIONA
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The objective of this study was to detect and characterize by nuclear magnetic resonance nmr and optical spectroscopy and by X-ray crystallographic study structural differences between non-aged and aged organophosphotyl OP conjugates of acetylcholinesterase AChE and chymotrypsin Cht which might help to explain the unexpected resistance of the aged form to commonly used reactivators. During the period covered by this report we synthesized novel organosphosphate inhibitors of serine hydrolases, performed spectroscopic studies on the conformational stabilization of aged and non-aged OP-Cht and OP- AChE conjugates and investigated OP-Cht conjugates by 31 P-nmr spectroscopy. In addition, we prepared crystalline OP-Cht conjugates and determined their structure by X-ray crystallography.